<p>I am creating my application using Django, and am wondering how I can make Django use my CSS file? What settings do I need to do to make Django see the css file?</p>

<p>NB: On a local machine</p>

What settings do i need to do to make Django see the css file?
None.
Make sure your template includes the CSS file (as standard HTML does) and put the CSS file on the media server.
To clarify: With Django it is highly recommended that you serve all your media (everything that isn't dynamic html) from a different server instance. How you implement that is completely up to you but most people create a subdomain.