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ZymoBIOMICS Mock Community — MinION POD5
Raw Oxford Nanopore signal data (POD5) from a ZymoBIOMICS mock microbial community, sequenced as a test run. No human or patient material: the sample is a commercially available defined-composition standard.
Published as a teaching and benchmarking dataset for biohub and the Griffin-Pipeline.
Run
| Instrument | Oxford Nanopore GridION (position X5) |
| Flow cell | FLO-MIN114 (R10.4.1), ID FBG46173 |
| Kit | SQK-RBK114-96 (rapid barcoding) |
| Run date | 2026-08-24, 3 h target runtime |
| Basecalling (on instrument) | Super-accurate v5.2.0, 400 bps |
| Barcodes observed | 11 in fastq_pass |
Files
| File | Size | Notes |
|---|---|---|
FBG46173_23a27d2f_aa1ff4e9_0.pod5 |
1.4 GB | |
FBG46173_23a27d2f_aa1ff4e9_1.pod5 |
2.2 GB | |
FBG46173_23a27d2f_aa1ff4e9_2.pod5 |
2.7 GB | |
FBG46173_23a27d2f_aa1ff4e9_3.pod5 |
403 MB | smallest; use this one for demos |
report_FBG46173_20260824_1016_23a27d2f.html |
1.3 MB | MinKNOW run report |
The POD5 files are raw squiggle, not basecalls. Expect to run Dorado.
For a workshop or a quick test, take _3.pod5 alone — it exercises the full
pipeline at roughly a sixth of the basecalling cost.
Note on the run report
The instrument serial in the MinKNOW report has been replaced with GXB00000.
Nothing else in the file was modified.
Usage
hf download TheBioHub/zymo-mock-minion-pod5 --repo-type dataset --local-dir ./zymo
Single file:
hf download TheBioHub/zymo-mock-minion-pod5 \
FBG46173_23a27d2f_aa1ff4e9_3.pod5 \
--repo-type dataset --local-dir ./zymo
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